FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14674452
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167550.11417802859009658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC215350.019.416284
CCAAAGT259600.018.501362
AAGTGCT248100.017.083235
CCCAAAG325800.016.9628161
CAAAGTG267400.016.704393
GCTGGGA409050.015.3857029
CTACTCG33050.014.8994712
AGTGCTG309300.014.1647576
GCTACTC94550.013.2910471
TACTCGG44500.012.6673213
GTGCTGG401400.011.732417
TAGCTGG148200.011.6516617
TGCTGGG462950.011.3596768
AGTAGCT127250.011.2706395
CCTATAT61000.011.2369771
GCTACTT102750.010.7015571
GGAGGCT306100.010.5365939
CTACTTG100900.010.1850832
GCTATAC37800.010.0113391
GCTATAT58050.09.8400161
Produced by FastQC (version 0.11.5)