FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14504765
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC209400.018.3912814
CCAAAGT255950.017.0525652
CCCAAAG317900.016.181521
AAGTGCT250100.015.512445
CAAAGTG268250.015.3933713
GCTGGGA402750.014.9278559
CGTCGAG22500.014.2693631
ACGTCGA17750.013.6664321
CTACTCG31950.013.6160892
CGAGTAG34000.013.4244323
AGTGCTG307250.013.3694496
GCTACTC90150.012.58363151
CCTATAT58600.011.931721
TAGCTGG143000.011.4211767
GTGCTGG392550.011.3552437
CCGAGTA42100.011.0109462
AGTAGCT131550.010.7884035
TGCTGGG458550.010.6539158
TACTCGG41400.010.5081053
GAGTAGC91200.010.4786394
Produced by FastQC (version 0.11.5)