FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14253689
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC204400.019.3814754
CCAAAGT254200.017.3437712
CCCAAAG308000.016.5271281
AAGTGCT246050.016.447635
CAAAGTG260050.016.2985133
GCTGGGA415350.015.7943029
CTACTCG34850.014.8712512
TACTCGG41200.014.64711953
CGTCGAG20150.014.093341
AGTGCTG313550.013.3598516
TAGCTGG148350.013.2085497
AGTAGCT133800.012.6291075
GCTACTC91750.012.2258761
CGAGTAG32200.011.9060693
GTGCTGG402450.011.2549177
GGAGGCT308600.010.9509989
CCTATAT58900.010.8481621
TGCTGGG460400.010.8407968
CCGAGTA41550.010.4228452
GAGTAGC92200.010.3952854
Produced by FastQC (version 0.11.5)