FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13846137
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG33400.019.3596272
AAAGTGC197950.019.3402024
CCAAAGT241250.017.8487322
AAGTGCT233200.016.8119415
CCCAAAG305650.016.2238641
CAAAGTG258500.016.0225473
TACTCGG40850.015.3043243
GCTGGGA398200.015.1624349
GCTACTC91800.015.0350531
AGTGCTG301600.013.4941246
CGAGTAG30850.013.3566133
AGTAGCT122450.012.6445985
TAGCTGG139250.012.2932537
GTGCTGG386950.011.4911777
GGAGGCT301300.011.43388759
GCTATAT54300.011.3989631
GCTATAA64600.011.2334611
CTACTTG94200.011.0129592
GAGTAGC84600.010.7465084
TCGGGAG74100.010.7354766
Produced by FastQC (version 0.11.5)