FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13846137
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC205050.018.2124394
CCAAAGT246450.016.794892
ACGTCGA19150.016.3130591
AAGTGCT238700.015.9426025
CAAAGTG257050.015.5774853
CGTCGAG21600.015.44883251
CCCAAAG317150.015.2228411
CTACTCG32150.014.7955552
GCTGGGA406100.014.7214659
GCTACTC89300.013.9930611
AGTGCTG298550.013.2459596
TACTCGG38950.013.1238393
TAGCTGG142500.012.7057997
CGAGTAG30650.012.276693
TGCTGGG440800.011.1786618
CCTATAT56900.011.1052741
GTCGAGA32000.011.09357551
GTGCTGG384500.011.0613247
AGTAGCT127700.010.9527245
CTACTTG93450.010.33232
Produced by FastQC (version 0.11.5)