FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14253689
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158520.11121331467243323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC203000.019.5557564
CCAAAGT250450.017.3339942
CTACTCG35200.017.157532
AAGTGCT240650.016.495425
CAAAGTG258300.016.1140143
CCCAAAG315200.015.6389091
GCTGGGA409700.014.75196559
TACTCGG43850.014.2542243
GCTACTC95850.013.7290051
TAGCTGG143050.013.2056177
AGTGCTG307000.012.9308226
AGTAGCT126550.012.1207375
GAGTAGC90000.011.0474
GGAGGCT306950.011.0135069
GCTATAC39200.010.8874121
GGAGGTT156750.010.8288218
GTGCTGG405700.010.6779967
GCTAATT101350.010.5275421
TGCTGGG459750.010.2728458
CGAGTAG34750.010.2198773
Produced by FastQC (version 0.11.5)