FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14317640
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164100.11461386094356331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC203750.020.0804044
CTACTCG33000.018.7292212
CCAAAGT254100.018.5083522
CCCAAAG308550.017.7831271
AAGTGCT240150.016.9480465
CAAAGTG265250.016.5218683
GCTGGGA415950.015.8138349
TACTCGG43250.015.7657463
GCTACTC93050.015.5058651
CGAGTAG30700.014.8071353
CCGAGTA35250.013.50299552
AGTGCTG305750.013.4744386
AGTAGCT126450.013.3130375
TAGCTGG153100.013.1764487
GAGTAGC86250.012.18849854
GTGCTGG396600.011.4267627
GGAGGCT314550.011.15599
GCTACTT100050.010.8690271
TGCTGGG463050.010.861118
GCTATAT56150.010.6327561
Produced by FastQC (version 0.11.5)