FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13797172
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC183500.017.0073744
CCAAAGT221600.015.9702842
AAGTGCT216550.014.8322275
CAAAGTG235400.014.1167513
CCCAAAG298350.013.6382521
GCTGGGA445800.012.3161099
AGTAGCT117900.011.6077765
CGAGTAG36200.011.5711623
CTACTCG36550.011.4587782
AGTGCTG305250.011.3239316
TAGCTGG140500.011.2339727
GCTACTC92500.011.0160861
GAGTAGC86800.010.858754
GCTAATT80100.010.1074521
GCTACTT88250.09.727621
CCGAGTA44550.09.7144552
CTACTTG82800.09.5262632
TACTCGG46000.09.2577683
GCTATAG41750.09.0272081
GGAGGCT348750.08.9332959
Produced by FastQC (version 0.11.5)