FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13487286
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC176950.017.166724
CCAAAGT210750.015.2029462
AAGTGCT210950.014.5668915
CAAAGTG225250.014.1650933
CGAGTAG36200.013.3078713
CCCAAAG298350.013.2624471
AGTAGCT112350.012.8674065
GCTGGGA428750.012.43524559
TAGCTGG136900.011.94111257
GAGTAGC85450.011.5227374
AGTGCTG289800.011.424446
CTACTCG36050.011.4242942
GCTATAG40200.011.11016751
GCTATAT41050.010.8801151
GCTACTC85800.010.6549331
CCGAGTA44800.010.283682
GCTAATT77100.09.8659481
TACTCGG44750.09.6731223
GCTAATA36800.09.4817581
CTGTCTA87050.09.177681145
Produced by FastQC (version 0.11.5)