FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13797172
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC177450.016.2871784
CCAAAGT219700.014.9663912
CGTCGAG30050.014.4114091
AAGTGCT213200.013.7525615
CAAAGTG230800.013.460023
CCCAAAG290700.013.3594621
GTCGAGA32650.013.2637931
ACGTCGA19200.013.0966581
CTACTCG35150.011.7179832
AGTAGCT115050.011.5289155
TAGCTGG141400.011.1578497
GCTGGGA442150.011.0364819
GCTATAT39350.011.0054091
AGTGCTG302550.010.6602236
GCTAATT81700.010.4303051
CGAGACT64000.010.2590491
CTACTTG77400.09.6509272
GCTACTT86750.09.5815711
TCGAGAC50150.09.4658463
GCTACTC86800.09.3346381
Produced by FastQC (version 0.11.5)