FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13487286
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA21750.019.9091241
AAAGTGC179800.017.0071624
CGTCGAG31550.016.160071
CCAAAGT216550.015.7007282
GTCGAGA33650.014.9381372
AAGTGCT212900.014.3958175
CAAAGTG228500.013.93240453
CCCAAAG294900.012.836431
AGTAGCT109500.012.2414975
CTACTCG36300.011.7320052
CGAGACT67300.011.4155631
TAGCTGG133500.011.3481717
AGTGCTG295850.011.2090666
GCTGGGA442300.011.080719
GCTAATT78300.010.6146321
GCTATAT38500.010.5217271
TACTCGG45200.010.5031093
TCGAGAC50650.010.4756653
GCTACTC85850.010.2506161
GACTTGT107050.09.326033
Produced by FastQC (version 0.11.5)