FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13961525
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC183500.016.281114
CCAAAGT219950.015.3245952
AAGTGCT219400.013.9680865
CTACTCG34750.013.65588952
CAAAGTG237700.013.47771553
CCCAAAG297700.012.5428221
GCTACTC88350.012.2446241
TAGCTGG137600.011.315777
GCTGGGA449350.011.2829939
AGTAGCT108650.011.2439095
CGAGTAG35650.011.1595873
GCTAATT77100.011.1345091
TACTCGG44900.010.5704953
AGTGCTG305050.010.3445646
GAGTAGC85750.010.0940144
GGAGGCT344950.09.5151179
CCTATAG42600.09.0110081
CCTAAAT67000.08.8545071
TTGGGAG220750.08.7603996
CCGAGTA44200.08.6837292
Produced by FastQC (version 0.11.5)