FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13772162
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG27950.016.4726941
AAAGTGC181750.015.771414
CCAAAGT219000.014.6492462
AGTAGCT118750.014.3891665
GTCGAGA34400.013.7864382
AAGTGCT215550.013.52481655
TAGCTGG145650.013.2155727
CAAAGTG235250.012.896233
CCCAAAG297100.012.56185151
CTACTCG36700.012.5423652
CGAGACT64200.011.8438791
GCTGGGA445400.011.7904989
GAGTAGC89700.011.5072734
GCTACTC86950.011.4727871
CGAGTAG38700.010.9931473
GCTAATT83150.010.9064551
GTAGCTG164250.010.5729726
AGTGCTG303150.010.4448316
TCGAGAC51400.010.3122553
TACTCGG44350.010.0644383
Produced by FastQC (version 0.11.5)