FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13772162
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC178450.017.7794254
CCAAAGT217450.016.0272852
AAGTGCT213850.015.1991015
CAAAGTG231250.014.4423273
CCCAAAG300850.013.9473811
GCTGGGA445450.012.4241899
AGTAGCT114000.011.8084725
GCTAATT76700.011.177791
AGTGCTG303600.011.1206616
TAGCTGG143900.010.9087917
CTACTCG37400.010.6231332
GAGTAGC86100.010.6094054
CGAGTAG37750.010.5278893
CTACTTG78500.010.4775962
GCTACTC86550.010.3888821
GCTACTT88600.010.3845191
GCTATAT40450.010.3390091
TACTCGG42750.010.1120453
CCGAGTA42400.09.6991912
CCTATAT42850.09.5972611
Produced by FastQC (version 0.11.5)