FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17739952
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC296950.020.917654
CGAGTAG51950.020.5357113
CCAAAGT354350.019.6288742
AAGTGCT336450.018.6081585
CCCAAAG437200.017.9159341
CAAAGTG369750.017.9000913
AGTAGCT197150.017.7293015
CCGAGTA60000.017.5466482
GCTGGGA601500.017.3986079
TAGCTGG229450.016.7022387
GAGTAGC136050.016.5083774
CTACTCG50550.016.2449682
AGTGCTG422600.015.1802676
GTAGCTG238300.014.7857186
GCTACTC131650.014.7677481
TACTCGG58850.014.0730193
CCCGAGT99600.012.6138911
GTGCTGG553700.012.2327157
TGCTGGG619700.011.9492618
GGAGGCT432050.011.6644159
Produced by FastQC (version 0.11.5)