FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17698219
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC281450.019.8717124
CCAAAGT344000.018.8491482
CGAGTAG53650.018.5736313
AGTAGCT192600.018.1084295
CCCAAAG421400.017.4353871
AAGTGCT328750.017.2688485
CAAAGTG355000.017.2371853
TAGCTGG228700.017.1199327
GCTGGGA594000.017.1166849
CCGAGTA60300.016.9907952
CTACTCG53200.016.6202342
GAGTAGC137200.015.6511224
GCTACTC140300.015.3553641
GTAGCTG235400.015.3527986
TACTCGG63850.014.617363
AGTGCTG407300.014.6106066
CCCGAGT100900.013.1446811
GGAGGCT444750.012.5110849
TTGGGAG337100.012.1574056
GTGCTGG543550.011.8763327
Produced by FastQC (version 0.11.5)