FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18200587
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC289450.022.2463634
CCAAAGT358350.019.4194372
AAGTGCT339600.019.1888755
CAAAGTG366000.018.8980063
CGAGTAG52300.018.5284143
CCCAAAG443150.018.0371571
GCTGGGA605850.017.7342769
CTACTCG51650.017.4024372
AGTAGCT201150.017.3851535
GCTACTC138200.017.026031
TAGCTGG229650.016.5736277
CCGAGTA59500.016.5227172
GAGTAGC140800.015.6599454
AGTGCTG429400.015.2579426
TACTCGG60650.014.819723
GTAGCTG244050.014.7609696
GGAGGCT448650.012.2870919
GTGCTGG560850.012.2329887
GCTACTT138950.011.9297141
TTGGGAG334150.011.8526116
Produced by FastQC (version 0.11.5)