FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18475258
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC291750.020.7403564
CGAGTAG52550.019.3694043
CCAAAGT360100.019.3184152
AAGTGCT336350.018.5537855
AGTAGCT198250.017.8105345
CCCAAAG446750.017.7769221
GCTGGGA616850.017.6275869
CAAAGTG368750.017.5136533
CTACTCG53250.017.1373922
TAGCTGG241100.016.2754577
CCGAGTA62200.015.9129132
GCTACTC139550.015.598771
AGTGCTG427350.015.1451676
GAGTAGC139300.014.9666694
GTAGCTG251850.014.9120946
TACTCGG64150.014.8820663
GGAGGCT464150.012.5528039
GTGCTGG558900.011.9946587
TTGGGAG342500.011.9285956
TGCTGGG645900.011.5527828
Produced by FastQC (version 0.11.5)