FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20069927
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC293400.018.027854
CCAAAGT359050.016.1829912
CTACTCG56800.015.9956222
AGTAGCT195500.015.7794345
CGAGTAG55250.015.5520373
AAGTGCT336150.015.462775
CAAAGTG372500.015.1259783
TAGCTGG242200.014.8589867
GCTGGGA645350.014.7994199
CCCAAAG465150.014.3994951
CCGAGTA66150.014.0541262
TACTCGG68600.013.7575293
GCTACTC147000.013.4632131
GAGTAGC139200.013.1046264
AGTGCTG440650.012.4830996
GTAGCTG253250.012.2646266
GCTACTT147250.011.766291
GCTATAT71100.011.2926271
TTGGGAG352600.010.8263516
GGAGGCT486550.010.6110129
Produced by FastQC (version 0.11.5)