FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19370775
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC284800.018.4882374
CCAAAGT349300.016.7060832
CGAGTAG50100.016.136293
CTACTCG53300.016.0926572
AAGTGCT337450.015.9379945
AGTAGCT181800.015.8551985
CAAAGTG361000.015.5590523
GCTGGGA605950.015.308689
CCCAAAG448650.015.0689271
GCTACTC138400.015.046041
TAGCTGG222050.014.8216567
CCGAGTA58700.014.3726982
TACTCGG63650.014.24737553
GAGTAGC135050.013.2739224
AGTGCTG425650.013.0663346
GTAGCTG239900.012.2804336
CTACTTG127900.012.1483372
GGAGGCT477700.011.9691979
GCTACTT139700.011.4816681
CGCGATA9400.001285623411.053084145
Produced by FastQC (version 0.11.5)