FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19135752
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC287100.019.241274
CTACTCG50250.017.506972
CCAAAGT343200.017.327922
AAGTGCT337750.016.4009365
CAAAGTG359450.016.1894653
CCCAAAG445400.015.4990041
CGAGTAG53700.015.069163
TACTCGG60050.014.9990053
AGTAGCT191750.014.7563845
GCTGGGA616650.014.7047399
GCTACTC142150.014.2716281
TAGCTGG225600.013.6662847
GAGTAGC131600.013.6358294
CCGAGTA62750.013.4587452
AGTGCTG427300.013.0959216
GTAGCTG245950.011.9341376
CTATTCG18850.011.5741032
GGAGGCT467000.011.0199769
CTACTTG127200.010.955072
GTGCTGG584100.010.4483397
Produced by FastQC (version 0.11.5)