FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19370775
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC274900.017.8119094
CCAAAGT337700.016.31162
AAGTGCT329550.014.9008565
CAAAGTG354000.014.8453723
AGTAGCT189650.014.76411155
CTACTCG49950.014.5066672
CCCAAAG442150.014.46346951
GCTGGGA606100.014.3126679
TAGCTGG222350.013.510117
CGAGTAG51300.013.4394793
CCGAGTA60050.012.8868152
GAGTAGC133200.012.7288394
TACTCGG61050.012.6759573
GCTACTC140600.012.0589621
AGTGCTG425850.011.8781966
GTAGCTG238450.011.7130876
GCTACTT137150.011.1825011
GGAGGCT468750.010.8367169
CTACTTG130700.010.7651562
TTGGGAG340700.010.2833696
Produced by FastQC (version 0.11.5)