FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19367626
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC284450.019.473654
CCAAAGT347550.017.7403872
AAGTGCT338500.016.8633585
CAAAGTG365800.016.2587473
CCCAAAG444650.015.9565391
CTACTCG53800.015.8299442
GCTGGGA617500.015.4796649
AGTAGCT188350.015.3214595
CGAGTAG49650.014.6013173
TAGCTGG231400.014.4786017
TACTCGG63700.014.4745783
GCTACTC141350.013.8932821
AGTGCTG440100.013.6740686
GAGTAGC132600.012.79244
GTAGCTG243850.012.3538986
CCGAGTA61000.011.8845762
GGAGGCT473150.011.6929049
GCTATAT70200.011.4304731
CCTATAT78050.010.8219331
GTGCTGG595200.010.7966977
Produced by FastQC (version 0.11.5)