FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19135752
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC281550.018.4835834
CCAAAGT343500.016.954022
AAGTGCT327050.016.407385
CAAAGTG360000.015.8835423
CGAGTAG49950.015.6488113
CCCAAAG439000.015.4956921
AGTAGCT191950.015.1516645
TAGCTGG222150.015.0572517
GCTGGGA607500.015.0119349
CTACTCG52000.014.4901972
AGTGCTG423900.013.356456
GCTACTC135700.013.1292241
GAGTAGC136800.012.7662344
CCGAGTA61500.012.70988752
TACTCGG64400.012.6842843
GTAGCTG247100.012.197466
GGAGGCT468350.010.9163959
GTGCTGG571200.010.6148387
GCTACTT140450.010.5793521
GCTAATT148700.010.5133321
Produced by FastQC (version 0.11.5)