FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19367626
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC283850.020.07654
CCAAAGT347500.018.3205992
CTACTCG51750.017.807662
AAGTGCT335100.017.1340625
CCCAAAG449300.016.5546571
CAAAGTG364600.016.5541763
CGAGTAG51050.016.5356943
AGTAGCT190650.016.4973935
GCTGGGA616300.015.6549879
TAGCTGG233150.015.5732037
GAGTAGC135850.014.2418014
TACTCGG65850.013.994343
CCGAGTA59400.013.9746682
AGTGCTG430600.013.6609636
GCTACTC136000.013.6598721
GTAGCTG246350.013.0816356
GGAGGCT477000.011.3010249
GTGCTGG579050.011.2041417
TGCTGGG665700.010.7289678
TTGGGAG334600.010.5146096
Produced by FastQC (version 0.11.5)