FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21576429
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG68900.024.9622143
CCGAGTA78550.022.6905272
CTACTCG70350.021.7864192
TACTCGG76850.021.1165333
AGTAGCT264500.020.5821425
AAAGTGC372000.020.0406654
GAGTAGC180400.019.182744
TAGCTGG313550.019.1097747
GCTGGGA713450.019.00369
CCAAAGT453050.018.6142332
GTAGCTG301850.018.2191726
AAGTGCT417450.018.0746975
GCTACTC185900.017.3905451
CAAAGTG461850.017.298253
CCCAAAG534050.017.277321
CCCGAGT126250.016.045681
AGTGCTG494300.015.5592096
GCTACTT175650.014.4952121
GGAGGCT536600.014.3975039
TTGGGAG441000.014.3417746
Produced by FastQC (version 0.11.5)