FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21001247
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG61850.023.7444783
CCGAGTA69050.022.3674562
AAAGTGC361200.021.541154
CCAAAGT429550.020.4124622
CCCAAAG501050.019.6744081
AAGTGCT399500.019.5833285
AGTAGCT253550.019.5138915
GCTGGGA699300.019.3984979
TAGCTGG300550.018.7260387
CTACTCG63350.018.476322
CAAAGTG449600.018.366083
GAGTAGC171400.018.0689754
GTAGCTG283750.017.7068566
TACTCGG71850.017.547333
GCTACTC176200.016.70941
AGTGCTG474400.016.6240526
CCCGAGT116500.016.5903721
GGAGGCT533550.014.823529
GCTACTT171950.014.7856951
TTGGGAG430550.014.6955736
Produced by FastQC (version 0.11.5)