FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18849521
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC278450.018.5121294
CCAAAGT333300.016.472852
CTACTCG49750.016.4217642
AAGTGCT323000.015.700865
CCCAAAG428250.015.3336271
CAAAGTG349350.015.23502353
CGAGTAG48000.014.56323053
GCTACTC130850.014.50476551
TACTCGG60150.014.4981463
AGTAGCT174500.014.3131025
GCTGGGA575600.014.0532139
CCGAGTA56200.013.18191052
TAGCTGG205250.013.0803727
AGTGCTG412150.012.6409616
GAGTAGC126200.011.8424554
GGAGGCT427050.011.2936939
GCTACTT126650.011.2119861
GTAGCTG223650.010.9824666
GCTAATT139200.010.6987511
GCTATAT71450.010.4702111
Produced by FastQC (version 0.11.5)