FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17956676
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC259150.017.9664
CCAAAGT313100.016.7134842
CTACTCG46650.016.0250872
CCCAAAG392250.015.2734831
CAAAGTG331250.015.248373
AAGTGCT301750.015.1331775
GCTGGGA530700.014.342569
AGTAGCT166150.014.1999735
CGAGTAG46900.013.7203943
TAGCTGG194350.013.7069227
TACTCGG57200.013.6690663
CCGAGTA54300.013.5124572
GCTACTC121900.012.840731
GAGTAGC121550.012.4076364
AGTGCTG386300.012.3231076
GTAGCTG215300.011.1838596
CCTATAT66450.011.1525381
GCTATAT67750.011.1431
GGAGGCT411150.010.4682289
TTGGGAG306850.010.1471216
Produced by FastQC (version 0.11.5)