FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18979793
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG29200.017.7896771
AAAGTGC288300.017.0295934
CGAGTAG48450.015.7215263
CCAAAGT347050.015.5629762
CTACTCG49200.014.9186962
AAGTGCT335650.014.8335845
CAAAGTG363700.014.2985653
AGTAGCT183050.014.2240065
CCCAAAG445500.014.0232441
GCTGGGA587150.014.0107729
CCGAGTA59250.013.206272
TACTCGG58750.012.8473773
GAGTAGC130750.012.3930124
GTCGAGA41350.012.3921412
TAGCTGG219050.012.2654727
AGTGCTG415850.012.2558936
GCTACTC133750.012.2210311
GCTATAT68800.011.5770811
CCTATAT73750.011.45743751
GCTACTT133150.011.1317211
Produced by FastQC (version 0.11.5)