FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18979793
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC281100.017.434764
CCAAAGT338850.016.243082
CTACTCG48300.015.7671922
AAGTGCT321850.015.5500925
CGAGTAG48200.015.3667713
CCCAAAG421750.015.1888071
CAAAGTG351250.014.9776323
CCGAGTA55700.014.5425142
GCTGGGA583800.014.4664579
AGTAGCT180000.014.3829265
TAGCTGG214500.013.6514387
GCTACTC129700.013.5622181
TACTCGG60050.013.4871043
GAGTAGC129100.012.4931874
AGTGCTG418350.012.2118636
GTAGCTG236600.011.2644846
GCTAATT139450.010.8261731
GGAGGCT439500.010.7421699
TTGGGAG317250.010.5610976
CCTATAT74550.010.4970631
Produced by FastQC (version 0.11.5)