FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20158602
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC301050.016.5698684
CCAAAGT369300.015.8946442
CGAGTAG46750.014.8156483
CTACTCG48600.014.5396272
CCCAAAG456500.014.4568891
CAAAGTG385600.014.2247983
AAGTGCT352550.014.0890975
GCTGGGA581200.014.0234029
CGTCGAG31350.013.84071
AGTAGCT195350.012.6417395
TAGCTGG219350.012.5662357
GCTACTC130200.012.5241681
TACTCGG57850.012.0938253
AGTGCTG443700.011.66776856
CCTATAT82300.011.6499651
CCGAGTA61850.011.4248322
GCTATAT77200.010.6971641
GAGTAGC134350.010.6233294
GGAGGCT438200.010.3775319
ACGTCGA25800.010.3078441
Produced by FastQC (version 0.11.5)