FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20158602
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC310350.017.6295574
CCAAAGT366150.016.4332162
AAGTGCT352250.015.5724845
CCCAAAG456800.015.41091251
CAAAGTG386250.015.1968653
CTACTCG49500.014.9824852
GCTGGGA585900.014.9059779
CGAGTAG48750.014.6381463
TACTCGG62950.013.2254913
TAGCTGG213400.012.9193297
AGTGCTG447900.012.7943536
AGTAGCT189400.012.744775
GCTACTC146050.012.6490771
CCGAGTA57100.012.4981942
GCTATAT77850.011.8651091
GAGTAGC130950.011.8613424
GGAGGCT436200.011.5348679
CTGTCTA146800.011.293607145
GTGCTGG567050.010.920947
TGCTGGG642800.010.5056518
Produced by FastQC (version 0.11.5)