FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20766707
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC321250.016.8514674
CTACTCG50550.015.7736032
CCAAAGT385050.015.6943672
CCCAAAG470250.015.0991111
AAGTGCT368100.014.7637655
GCTGGGA620000.014.5864599
CAAAGTG397900.014.484263
GCTACTC138750.013.7135541
CGAGTAG51200.013.6607043
TAGCTGG226000.013.3386177
AGTAGCT194400.013.3121285
CGTCGAG28500.013.0090371
CCGAGTA63250.012.7169952
TACTCGG61800.012.6757173
AGTGCTG455700.012.4015036
GAGTAGC135600.012.1212674
GTCGAGA43450.011.4309611
GGAGGCT452600.010.9874799
GTAGCTG246750.010.9130356
GCTAATT159750.010.8160891
Produced by FastQC (version 0.11.5)