FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20261791
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC312950.018.2827054
CCAAAGT380350.016.666122
CTACTCG49550.016.3796312
CCCAAAG459850.015.9975431
AAGTGCT358550.015.6459835
CAAAGTG393750.015.4899683
GCTGGGA590700.015.0679779
TACTCGG61250.013.7034453
CGAGTAG49650.013.1014183
AGTGCTG447450.013.0553596
GCTACTC143150.013.0552951
AGTAGCT192650.012.7443095
TAGCTGG222800.012.1843097
GTGCTGG569450.011.3404557
CCGAGTA60250.011.2643062
CCTATAT81800.011.1238941
GGAGGCT453200.010.9777799
TGCTGGG648300.010.8256068
GCTACTT146050.010.7832021
GCTAATT155050.010.7441471
Produced by FastQC (version 0.11.5)