FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20457776
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC301850.017.450774
CCAAAGT377050.015.769852
AAGTGCT349250.015.2225695
CGAGTAG49750.015.0452483
CCCAAAG466350.014.9285421
GCTGGGA589650.014.6753449
CAAAGTG386150.014.6374013
CTACTCG48800.013.9046322
CCGAGTA59550.013.6264842
AGTAGCT194150.013.5115345
TAGCTGG223700.012.7900227
TACTCGG62100.012.7290363
GCTACTC133050.012.7264371
GAGTAGC135700.012.2689644
AGTGCTG445600.011.9938596
GTCGAGA43700.011.2078661
GTAGCTG240100.010.9839856
GCTACTT145400.010.5386741
GTGCTGG562150.010.4405587
GCTAATT160650.010.3657931
Produced by FastQC (version 0.11.5)