FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20457776
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC314000.017.3536644
CTACTCG47250.017.3242952
CCAAAGT381500.015.8816222
CGAGTAG47300.015.8241283
AAGTGCT362750.015.0601435
GCTGGGA596100.014.8464539
CAAAGTG390550.014.8303323
CCCAAAG475700.014.5648581
AGTAGCT193850.014.0007545
CCGAGTA59400.013.8984722
TAGCTGG216550.013.8913887
TACTCGG58950.013.8835083
GCTACTC145000.013.2729951
GAGTAGC132700.012.967244
AGTGCTG452250.012.2504576
GCTAATT157550.011.5938141
GGAGGCT446850.011.1472969
GCTATAT75150.011.0821041
GTAGCTG244100.011.0617856
GTGCTGG570500.010.79101857
Produced by FastQC (version 0.11.5)