FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20492703
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC302800.016.4753784
CGAGTAG51450.015.5249693
CCAAAGT360500.015.2185072
GCTGGGA601300.015.0317599
AGTAGCT196400.014.7339735
AAGTGCT344850.014.6899185
CCCAAAG445150.014.6594911
CCGAGTA62200.014.1937032
CAAAGTG383000.014.0865053
TAGCTGG231250.013.7860847
CTACTCG53950.013.2472052
GCTATAT78150.012.8257461
GAGTAGC137700.012.771774
CGTCGAG30450.012.2001211
GTAGCTG249300.012.1977226
AGTGCTG441550.011.9964626
TACTCGG64750.011.1457353
GCTAATT158750.010.8175251
GCTACTC143850.010.5249361
GTCGAGA43900.010.3782571
Produced by FastQC (version 0.11.5)