FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19838690
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC334700.019.55674
CCAAAGT395850.018.0108382
GCTGGGA606300.017.8532669
CCCAAAG481250.017.5691471
CTACTCG52450.017.5572052
CGAGTAG50000.017.5492763
AAGTGCT385250.017.4042135
CAAAGTG411050.017.0250193
TACTCGG61500.016.2558293
TAGCTGG239800.015.7834117
GCTACTC149200.015.6880121
CCGAGTA57850.015.6696062
AGTAGCT212400.015.4451265
GAGTAGC141500.015.3010064
AGTGCTG451700.015.0679796
GGAGGCT442800.013.3767219
GTAGCTG252050.013.2447956
GTGCTGG541600.013.2193097
TGCTGGG626150.012.389858
GCTATAT91150.012.167951
Produced by FastQC (version 0.11.5)