FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19832114
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG51200.023.0483782
AAAGTGC326400.019.7966944
TACTCGG64200.018.8295843
CCAAAGT385300.018.301792
AAGTGCT376500.017.6020095
CCCAAAG466900.017.5424391
CGAGTAG50350.017.4351963
GCTGGGA610300.017.0487049
CAAAGTG408250.016.9556853
GCTACTC146500.016.5563221
CCGAGTA58550.015.4849612
TAGCTGG240700.015.0070467
AGTAGCT211250.014.9873055
AGTGCTG443950.014.8142516
GAGTAGC141650.014.6807344
GTAGCTG251800.013.0023926
GTGCTGG545300.012.9053847
GGAGGCT455750.012.6781349
TCGGGAG109450.012.4254916
ACTCGGG103050.012.3592394
Produced by FastQC (version 0.11.5)