FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20015157
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG53100.022.4954192
TACTCGG63250.019.5678943
AAAGTGC328450.018.5562534
CCAAAGT391950.017.3492872
GCTGGGA617300.017.309859
GCTACTC151400.017.2636051
CCCAAAG478800.016.436781
CGAGTAG49150.016.3972383
AAGTGCT382150.016.3817585
TAGCTGG243650.016.2132137
CAAAGTG409400.015.8362793
AGTAGCT218650.015.6979885
CCGAGTA56750.014.8354422
AGTGCTG457100.014.2781246
GTAGCTG253650.013.9574266
GGAGGCT460850.013.8492799
ACTCGGG103250.013.5900394
GAGTAGC144700.013.3273094
TCGGGAG115150.012.8104696
GCTACTT158100.012.4787031
Produced by FastQC (version 0.11.5)