FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19985842
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG55700.020.4092582
AAAGTGC330300.019.3230744
CCAAAGT399300.017.8386172
TACTCGG64200.017.5963483
CGAGTAG53100.017.3449633
GCTGGGA619250.017.0416979
CCCAAAG474300.016.7330841
CCGAGTA58650.016.5611722
AAGTGCT387700.016.5572765
CAAAGTG411700.016.5336283
GCTACTC150050.016.0163671
TAGCTGG243050.015.5162597
AGTAGCT211650.014.960785
AGTGCTG460250.014.3068476
GAGTAGC142000.014.1883154
GGAGGCT468250.014.1126879
GCTACTT160600.013.620141
GTAGCTG247600.013.2825716
CCCGAGT95300.012.9864091
CTACTTG153200.012.6803232
Produced by FastQC (version 0.11.5)