FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19832114
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC328150.019.40234
CTACTCG52450.019.2027662
CCAAAGT393500.018.1179982
CGAGTAG50500.018.0925673
GCTGGGA608450.016.9556229
AAGTGCT380150.016.922935
CCCAAAG473200.016.9214251
TACTCGG63100.016.6460553
GCTACTC141700.016.5662651
CAAAGTG411500.016.3117163
TAGCTGG239850.016.0834167
AGTAGCT208300.015.580465
CCGAGTA59850.015.2658442
AGTGCTG448850.014.5097186
GAGTAGC142850.014.1013334
GTAGCTG247850.013.5303386
GGAGGCT451250.012.8590929
CTACTTG147500.012.4373952
GTGCTGG557100.012.38907057
GCTACTT159650.011.9072621
Produced by FastQC (version 0.11.5)