FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20711100
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG60900.022.405572
CGAGTAG54350.020.6131673
TACTCGG67950.019.4467953
AAAGTGC358700.018.7998264
CCGAGTA63500.018.3214972
GCTACTC167050.017.888481
CCAAAGT424450.017.8503022
GCTGGGA650750.017.723569
TAGCTGG269600.017.3145857
AGTAGCT241100.017.24655
CCCAAAG501400.017.1918851
CAAAGTG439750.016.657663
AAGTGCT413100.016.6544365
GTAGCTG263050.015.752746
GGAGGCT489500.015.0086839
GAGTAGC154800.014.93841654
AGTGCTG467700.014.8329786
GCTACTT174600.013.7413321
ACTCGGG108150.013.2807664
CCCGAGT100100.013.1323851
Produced by FastQC (version 0.11.5)