FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20695627
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG57150.022.9940552
CGAGTAG54200.019.8731823
TACTCGG67150.019.6764723
GCTACTC158350.019.3696921
AAAGTGC354700.019.273134
CCGAGTA62550.018.483292
GCTGGGA638700.018.4607629
CCAAAGT424700.017.8381942
AGTAGCT240550.017.1349565
TAGCTGG268850.017.1206917
CCCAAAG502200.016.90651
GAGTAGC154150.016.8166754
AAGTGCT410850.016.7265035
CAAAGTG441100.016.7189433
GTAGCTG260300.015.8348986
AGTGCTG468150.015.03206356
GGAGGCT475150.014.3873099
CCCGAGT103150.013.857881
TCGGGAG114000.013.8578346
ACTCGGG107450.013.4327874
Produced by FastQC (version 0.11.5)