FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19009642
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC290500.019.3575024
CCAAAGT346400.018.3471342
CAAAGTG367150.016.8822653
AAGTGCT337350.016.863665
CTACTCG46850.016.7267172
CCCAAAG435000.016.662691
GCTGGGA565800.016.2368539
CGAGTAG44850.015.7119683
GCTACTC129050.014.8774021
AGTGCTG408950.014.2638046
CCGAGTA51200.014.0418352
TACTCGG59200.013.9682863
TAGCTGG211000.013.7383927
AGTAGCT183300.013.4582255
GCTATAT79450.012.2183831
GAGTAGC127900.012.1442834
GTGCTGG535300.011.8786837
TGCTGGG614250.011.6880228
CCTATAT82250.011.5401611
GTAGCTG219700.011.4256296
Produced by FastQC (version 0.11.5)