FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18790236
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC289750.018.465244
CTACTCG44350.017.999352
CCAAAGT349350.017.724332
CCCAAAG427500.016.2230511
CAAAGTG362950.015.9101483
AAGTGCT347900.015.7922665
GCTACTC124950.015.7763271
GCTGGGA569850.015.6340399
CGAGTAG44800.015.570383
TACTCGG52400.015.5078893
TAGCTGG207700.013.7786617
AGTGCTG409650.013.5522016
CCGAGTA52050.013.4022742
AGTAGCT178650.013.3238735
GAGTAGC123550.012.0485034
CCTAATA85450.011.9555051
GGAGGCT423350.011.7195239
GTAGCTG219650.011.6880626
CCTATAT82750.011.3023031
GTGCTGG525750.011.09187
Produced by FastQC (version 0.11.5)