FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18717151
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG43950.021.1081872
AAAGTGC296950.018.6474954
CCAAAGT356150.017.3856242
CGAGTAG44900.016.6573053
TACTCGG55900.016.3384063
AAGTGCT342450.016.3379615
CAAAGTG363900.016.2445453
GCTACTC128500.016.2375871
GCTGGGA569300.016.0806169
CCCAAAG429150.016.0278551
AGTAGCT185250.014.7128365
TAGCTGG209000.014.554917
CCGAGTA56650.013.9640962
AGTGCTG416900.013.9033226
GAGTAGC123100.013.3195614
GTAGCTG227000.012.2286046
CGTCGAG27350.012.1011631
GCTATAT78550.011.9991841
GGAGGCT420450.011.7847489
GCTAATT152300.011.62149051
Produced by FastQC (version 0.11.5)