FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18761042
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT227400.023.1749885
CGAGTAG90750.021.9302963
CCGAGTA102450.019.3624272
TAGCTGG299200.019.0137447
GAGTAGC182900.018.64834
GTAGCTG292300.018.1859786
CTACTCG81550.018.0629082
GCTACTC178000.016.7428321
AAAGTGC304300.016.3503444
TACTCGG93200.015.4534093
CCAAAGT349400.015.43950752
AAGTGCT325350.015.2119555
GCTGGGA747300.015.085089
CAAGTAG117700.014.6840223
CAAAGTG361700.014.6425783
CTACTTG113500.014.5356282
TTGGGAG425100.014.4297226
GCACTTT307500.014.2501211
GCTACTT120650.014.2236641
AAGTAGC121700.013.9861444
Produced by FastQC (version 0.11.5)