FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18785134
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT220000.023.9960235
TAGCTGG296500.019.5307147
CGAGTAG89200.019.502413
GAGTAGC174200.019.4865914
GTAGCTG285100.018.9547776
CCGAGTA99700.018.7569162
AAAGTGC315850.018.6370964
CCAAAGT360350.017.6191622
CTACTCG89600.017.3683872
GCTACTC177800.017.0983831
AAGTGCT344800.017.037175
CAAAGTG371250.016.4884913
CTGTCTA148250.016.100073145
GCTGGGA775650.016.0637519
CAAGTAG112700.015.5518633
TACTCGG102700.015.5378483
AAGTAGC120950.015.3037274
TTGGGAG406250.015.2668986
CTACTTG109500.015.1673662
CCCAAAG468600.015.0121831
Produced by FastQC (version 0.11.5)